Back issues No.4 - 1997 / Original Study  

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Comparison of positive rate in detecting Anti-Golgi Antibodies by indirect immunofluorescence using Hep-2 cells and IMR-33 cells as substrate
Hong-Shang Hong, Yea-Huey Chuang, Shao-Yei Liu, Jwo Farn Chiou
Dermatol Sinica 15: 236-241, 1997

In order to perform the related experiments concerning the Anti-Golgi Antibodies (AGA) or Golgi antigens, the way to detect and get AGA-positive sera is the most important thing. Previous authors used the animal tissue section as the substrate of the indirect immunofluorescence (IIF) to detect AGA, but the result is not satisfactory. To use the Hep-2 cells as substrate in IIF is quite popular now. There were authors used IMR-33 as substrate in indirect immunofluorescence and speculated the AGA wre more specific and sensitive to IMR-33 cell lines because they had a higher positive rate in our study is the same either use Hep-2 cells or IMR-33 cells as substrate. Because the choice of cell line as substrate in IIF will affect the detection rate directly, our experiment confirmed the suitability of Hep-2 cells as substrate in IIF test and it is not necessary to use other cell line as substrate because they do not show any special advantages.

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