Back issues No.3 - 2004 / Original Study  

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The Inhibitory Function of Shikonin on N-acetylation of 2-aminofluorene in Human Malignant Melanoma Cells (A375.S2)
Shang-Wen Yuan , Guang-Wei Chen , Jui-Lung Shen , Huei-Lin Chang , Jing-Gung Chung
Dermatol Sinica 22 : 201-207, 2004

Shikonin is one of the components of Lithospermum erythrorhizon (Chinese herb medicine) used for skin infection and allergy for many generations in the Chinese population. In this study, shikonin was used to determine the inhibition of N-acetylation of 2-aminofluorene (2-AF) in human malignant melanoma cell line (A375.S2). The amounts of N-acetylation and non-N-acetylation of 2-AF were measured by high performance liquid chromatography (HPLC). The results demonstrated that N-acetylation of AF from examined systems were decreased by shikonin in a dose-dependent manner. We also found out that shikonin inhibited the expression of NAT1 gene. Apparently shikonin inhibited N-acetylation of 2-AF in human malignant melanoma cell line. These are the first findings to show shikonin affect N-acetylation of 2-aminofluorene of human malignant melanoma cell line (A375.S2).

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