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Chemokine receptor CCR3 is important for migration of mast cells in neurofibroma
Wei-Chun Tai, Sin-Ting Wang, Chieh-Shan Wu, Tze-Yi Lin, Meng-Tse Wu
DERMATOLOGICA SINICA 28 (2010) 146–153

Background Neurofibroma consists of abundant extracellular matrix and many types of
cells, including Schwann cells (SCs), mast cells (MCs), fibroblasts and endothelial cells.
As SCs have been found to be the cell of origin for neurofibroma, how MCs may migrate
into the tumor has not been fully clarified. Given that chemokine receptor CCR3 is
found predominantly expressed by differentiated MCs, we postulated that CCR3 may
play a role in the homing of MCs to neurofibroma. The goal of this study is to investigate
the possible involvement of chemokine receptor CCR3 in the migration of MCs to
the neurofibroma.
Methods Expressional and functional assays for CCR3 and its ligands were performed
on MCs and SCs.
Results By real-time quantitative polymerase chain reaction and enzyme-linked immunosorbent
assay, we found one of the CCR3 ligand, CCL7 was highly expressed by
murine SC cell line SW10, and also moderately expressed by MCs. In serial chemotaxis
assays, MCs were found specifically responsive to CCL7 and also condition medium
from SW10 cells, indicating SCs may attract MCs by CCR3-mediated cell migration.
Conclusion The interaction of CCR3 and CCL7 may play important roles for MC migration
toward SC in the neurofibroma.

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